Journal: Scientific Reports

Article Title: Development of a recombinase polymerase amplification nucleic acid lateral flow assay for detecting Streptococcus suis serotype 2 in pork

doi: 10.1038/s41598-025-95480-2

Figure Lengend Snippet: Schematic representation of the RPA-NALFIA technique, illustrating the detection of RPA amplicons via immobilized capture antibodies on a nitrocellulose membrane.

Article Snippet: As expected, only S. suis DMST 26745 showed the RPA amplicon for the recN gene, and S. suis serotype 2 and 1/2 ( S. suis ATCC 43765 and DMST 26744) exhibited the amplicons for both the recN and cps2J genes on agarose gel electrophoresis.

Techniques: Membrane

Journal: Scientific Reports

Article Title: Development of a recombinase polymerase amplification nucleic acid lateral flow assay for detecting Streptococcus suis serotype 2 in pork

doi: 10.1038/s41598-025-95480-2

Figure Lengend Snippet: Detection specificity validation of RPA-NALFIA on 28 other bacterial species. ( A ) Visualization of RPA amplicons amplified from the recN and cps2J genes using agarose gel electrophoresis. ( B ) Visualization of RPA amplicons captured on lateral flow strips, targeting the recN gene on the T1 line and the cps2K gene on the T2 line.

Article Snippet: As expected, only S. suis DMST 26745 showed the RPA amplicon for the recN gene, and S. suis serotype 2 and 1/2 ( S. suis ATCC 43765 and DMST 26744) exhibited the amplicons for both the recN and cps2J genes on agarose gel electrophoresis.

Techniques: Biomarker Discovery, Amplification, Agarose Gel Electrophoresis

Journal: Scientific Reports

Article Title: Development of a recombinase polymerase amplification nucleic acid lateral flow assay for detecting Streptococcus suis serotype 2 in pork

doi: 10.1038/s41598-025-95480-2

Figure Lengend Snippet: Detection sensitivity and limit of detection validation of RPA-NALFIA using diluted gDNA of S. suis ATCC 43765. ( A ) Visualization of RPA amplicons from the recN and cps2J genes using agarose gel electrophoresis. ( B ) Visualization of RPA amplicons captured on lateral flow strips, targeting the recN gene on the T1 line and the cps2K gene on the T2 line. ( C ) Band intensity of recN on the T1 line of the lateral flow strips. (D) Band intensity of cps2J on the T2 line of the lateral flow strips.

Article Snippet: As expected, only S. suis DMST 26745 showed the RPA amplicon for the recN gene, and S. suis serotype 2 and 1/2 ( S. suis ATCC 43765 and DMST 26744) exhibited the amplicons for both the recN and cps2J genes on agarose gel electrophoresis.

Techniques: Biomarker Discovery, Agarose Gel Electrophoresis

Journal: Scientific Reports

Article Title: Development of a recombinase polymerase amplification nucleic acid lateral flow assay for detecting Streptococcus suis serotype 2 in pork

doi: 10.1038/s41598-025-95480-2

Figure Lengend Snippet: RPA-NALFIA results for gDNA extracted from 77 pork samples. RPA amplicons were captured on lateral flow strips, with the recN gene visualized on the T1 line and the cps2K gene visualized on the T2 line.

Article Snippet: As expected, only S. suis DMST 26745 showed the RPA amplicon for the recN gene, and S. suis serotype 2 and 1/2 ( S. suis ATCC 43765 and DMST 26744) exhibited the amplicons for both the recN and cps2J genes on agarose gel electrophoresis.

Techniques: